CD8-FITC/ CD4-PE/ CD3-PECy5, Mouse Monoclonal antibody-Human, Clone: UCH-T4/ Edu-2/ UCHT1
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Catalog numberACYT8F84PE93C4
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PricePlease ask
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Size1.5 ml
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Alternative name 1CD8-FITC/ CD4-PE/ CD3-PECy5, Mab anti-Human, Clone: UCH-T4/ Edu-2/ UCHT1
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Alternative name 2CD8-FITC/ CD4-PE/ CD3-PECy5, Mab antibody-Human, Clone: UCH-T4/ Edu-2/ UCHT1
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Alternative name 3CD8-FITC/ CD4-PE/ CD3-PECy5, Mab antibody-Homo sapiens, Clone: UCH-T4/ Edu-2/ UCHT1
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Alternative name 4anti-CD8-CD4-CD3- Clone: UCH-T4/ Edu-2/ UCHT1
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Alternative name 5CD8-CD4-CD3- Clone: UCH-T4/ Edu-2/ UCHT1
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional descriptionCD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolCD4, B3GNT4, CD8A, B4GALT4, ETV3, GALNT4, ERF, B3GALT4, UCHL1, SERPINB3
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Short nameCD8-FITC/ CD4-PE/ CD3-PECy5, Mouse Monoclonal antibody- , Clone: UCH-T4/ Edu-2/ UCHT1
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameCD8-fluorecein/ CD4 molecule-peroxidase/ CD3-PECy5, Mouse monoclonal (antibody to-)-H. sapiens, clonality: UCH-T4/ Edu-2/ UCHT1
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetCD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
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Gene info
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Identity
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Gene
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Long gene nameCD4 molecule
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Synonyms gene name
- CD4 antigen (p55)
- T-cell surface glycoprotein CD4
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
- C2-set domain containing
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameUDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-05-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 3-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-galactosyltransferase 4
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Synonyms gene name
- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 4-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameETS variant transcription factor 3
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Synonyms gene name
- ets variant gene 3, ETS family transcriptional repressor
- ETS variant 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-12-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepolypeptide N-acetylgalactosaminyltransferase 4
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Synonyms gene name
- UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Polypeptide N-acetylgalactosaminyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameETS2 repressor factor
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Synonyms gene name
- Ets2 repressor factor
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,3-galactosyltransferase 4
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Synonyms gene name
- UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4
- UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-12-01
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Entrez gene record
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Pubmed identfication
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Classification
- Beta 3-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L1
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Synonyms gene
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameserpin family B member 3
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Synonyms gene
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Synonyms gene name
- squamous cell carcinoma antigen 1
- serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3
- serpin peptidase inhibitor, clade B (ovalbumin), member 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-08-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data