CD4 FITC / CD8 PE / CD3 Cy5-PE[CD4 / CD8 PE / CD3 Cy5]

• Catalog number
  MBS396678
• Price
  Please ask
• Size
  NA

• Products_type
  Antibody
• Reactivity
  Human
• Description
  CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
• Properties
  Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Cy5.5, Anti-FITC Antibody

• Gene target
  CD4   CD8   CD3   PE[CD4   CD8   CD3  
• Gene symbol
  CD4, CD8A
• Short name
  CD4 FITC / CD8 PE / CD3 -PE[CD4 / CD8 PE / CD3 ]
• Technique
  FITC, Fluorescein
• Host
  Mouse
• Label
  FITC
• Alternative name
  CD4 molecule fluorecein / CD8 peroxidase / CD3 cyanine 5-peroxidase[CD4 molecule / CD8 peroxidase / CD3 cyanine 5]
• Alternative technique
  fluorescine
• Alternative to gene target
  CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098

Gene info

• Identity
  HGNC:1678
• Gene
  CD4
• Long gene name
  CD4 molecule
• Synonyms gene name
  • CD4 antigen (p55)
  • T-cell surface glycoprotein CD4
• GenBank acession
  • M35160
• Locus
  12p13.31
• Discovery year
  1986-01-01
• Entrez gene record
  920
• RefSeq identity
  • NM_000616
• Classification
  • CD molecules
  • C2-set domain containing
  • V-set domain containing
• VEGA ID
  OTTHUMG00000168514

Gene info

• Identity
  HGNC:1706
• Gene
  CD8A
• Long gene name
  CD8a molecule
• Synonyms gene
  • CD8
• Synonyms gene name
  • CD8 antigen, alpha polypeptide (p32)
  • T-cell surface glycoprotein CD8 alpha chain
• Locus
  2p11.2
• Discovery year
  1986-01-01
• Entrez gene record
  925
• Pubmed identfication
  • 1541829
• RefSeq identity
  • NM_001768
• Classification
  • V-set domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000130265
• Locus Specific Databases
  • CD8Abase: Mutation registry for CD8 deficiency
  • LRG_44

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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