2X PCR Taq Plus MasterMix
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Catalog numberG901
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Price:
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Size25.0 ml (1000 Rxns)
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Product categoryPCR, RT-PCR & qPCR
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ApplicationPCR. For others please contact us
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LiteratureRefer to NCBI PubMed for current references.
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Technical datasheetPlease contact us to receive a datasheet or any other useful technical documentation available.
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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PropertiesThermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Short name2X PCR Taq Plus MasterMix
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TechniquePCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. ABM PCR reagents TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
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Alternative name2X PCR test kit Taq Plus MasterMix
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Alternative techniquedna-amplification
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MeSH Data
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Name
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ConceptScope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data