ELISA test for Horse E3 ubiquitin-protein ligase Mdm2 (MDM2)

• Catalog number
  AE33934HO-48
• Price
  Please ask
• Size
  1x plate of 48 wells

• Description
  Horse E3 ubiquitin-protein ligase Mdm2 (MDM2) ELISA Kit is manufactured by highest quality antibodies and plates to provide you with excellent and reproducible results in your work. The specifically designed buffers will ensure optimal conditions in each step from diluting the samples, through the incubation to washing.
• Storage and shipping
  Store and ship all of of the comptents of the EIA assay for Horse E3 ubiquitin-protein ligase Mdm2 (MDM2) on blue ice/ice packs at +4 degrees Celcius. Afoid freezing and especially freeze-thaw cycles as such cycles may denaturate the peptide chains in the antibodies, standards and enzymes, thus reducing the activity and sensitivity of the kit.
• Advise tips
  Small volumes of the liquid components of the MDM2 ELISA Kit may get caught on the vials' walls and seals. Prior to use, briefly centrifuge the vials to ensure that all of the vial's content is on the bottom of the vial.
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
• Storage
  Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
• Group
  Enzyme-Linked Immunosorbent Assay kits

• Gene target
  for   ubiquitin-protein   ligase   Mdm2   MDM2  
• Gene symbol
  MDM2
• Short name
  MDM2 ELISA Kit
• Technique
  ELISA test, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests.
• Host
  Horse, antigen assay ELISA kit 1 Kit (96 Wells)
• Species
  Horse (Equus caballus; Equine), Horses
• Alternative name
  EIA assay for Horse E3 ubiquitin-protein ligase Mdm2 (MDM2)
• Alternative technique
  elisas, tests

Gene info

• Identity
  HGNC:6973
• Gene
  MDM2
• Long gene name
  MDM2 proto-oncogene
• Synonyms gene name
  • mouse double minute 2, human homolog of; p53-binding protein
  • Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
  • Mdm2 p53 binding protein homolog (mouse)
  • MDM2 proto-oncogene, E3 ubiquitin protein ligase
• Synonyms
  • HDM2
  • MGC5370
• Locus
  12q15
• Discovery year
  1993-12-10
• Entrez gene record
  4193
• Pubmed identfication
  • 1614537
  • 16905769
  • 29123033
• RefSeq identity
  • NM_002392
• Classification
  • Zinc fingers RANBP2-type
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000142827

MeSH Data

• Name
  Ligase Chain Reaction
• Concept
  Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
• Tree numbers
  • E05.393.620.311
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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