Mouse E3 ubiquitin-protein ligase Mdm2 (Mdm2)

Description

Recombinant Mouse E3 ubiquitin-protein ligase Mdm2(Mdm2) expressed in E.coli
Specifications

Uniprot ID: P23804; Host: Mus musculus (Mouse); Expression system: E.coli; Expression region: 1-489aa; Sequence information: Full Length; Tag: N-terminal 6xHis-tagged; Purity: Greater than 90% as determined by SDS-PAGE.
Additional_information

MW: 58.6 kDa; Buffer composition: Tris-based buffer with 50% glycerol.
Storage_and_shipping

Shipped on ice packs (+4°C). The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Repeated freezing and thawing should be avoided. Store working aliquots at 4°C for up to one week.
Notes

For research use only.
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus

Identity

HGNC:6973
Gene

MDM2
Long gene name

MDM2 proto-oncogene
Synonyms gene name
- mouse double minute 2, human homolog of; p53-binding protein
- Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
- Mdm2 p53 binding protein homolog (mouse)
- MDM2 proto-oncogene, E3 ubiquitin protein ligase
Synonyms
- HDM2
- MGC5370
Locus

12q15
Discovery year

1993-12-10
Entrez gene record

4193
Pubmed identfication
RefSeq identity
- NM_002392
Classification
- Zinc fingers RANBP2-type
- Ring finger proteins
VEGA ID

OTTHUMG00000142827

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data