Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometry*

  • Catalog number
    22904
  • Price
    Please ask
  • Size
    100 Tests
  • Description
    Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometry* is the kit that provides a sensitive, one-step fluorimetric assay to detect intracellular ROS in live cells with one hour incubation. It is optimized for flow cytometry applications.
  • Similar product
  • Shipping
    Room Temperature
  • Storage
    Freezer
  • Shelflife
    6 months
  • Chemical components
    Component A: Amplite™ ROS Green ; Component B: Assay Buffer ; Component C: DMSO
  • Excitation Wavelength
    490 nm
  • Emission Wavelength
    520 nm
  • Personal precautions
    Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
  • Uses
    Laboratory chemicals *For Research Use Only*
  • Technical data
  • Data sheet
  • Ordering
    To order Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometry*, please use the Cat. Nr.22904 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometry*
  • Test
    aat supplies other types of Assays as 1. Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
  • Additional description
    For cells, cell lines and tissues in culture till half confluency. FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
  • Gene target
    Meter   Fluorimetric   Intracellular   ROS   Activity   Kit   Optimized   for  
  • Gene symbol
    ROS1, GUCY2F, GUCY2D
  • Short name
    Meter Fluorimetric Intracellular ROS Activity Assay Kit*Optimized for *
  • Technique
    Flow Cytometry, intracellular, Assay, Flow, assays
  • Host
    Assay
  • Alternative name
    cellular Meter fluorimetry Intracellular Total ROS Activity test reagent*Optimized to measure Flow Cytometry*
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    cell, total
Gene info
  • Identity
    HGNC:10261
  • Gene
    ROS1
  • Long gene name
    ROS proto-oncogene 1, receptor tyrosine kinase
  • Synonyms gene name
    • v-ros avian UR2 sarcoma virus oncogene homolog 1
    • v-ros UR2 sarcoma virus oncogene homolog 1 (avian)
    • c-ros oncogene 1 , receptor tyrosine kinase
  • Synonyms
  • GenBank acession
  • Locus
    6q22.1
  • Discovery year
    1986-01-01
  • Entrez gene record
    6098
  • Pubmed identfication
  • Classification
    • Receptor tyrosine kinases
    • Fibronectin type III domain containing
  • VEGA ID
    OTTHUMG00000016188
  • Locus Specific Databases
Gene info
Gene info
MeSH Data
  • Name
    Flow Cytometry
  • Concept
    Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
Filters
Meter Fluorimetric Intracellular ROS Activity Assay Kit Opti
Contact
Back
Chat with gentaur.com employee