PTEN, human recombinant

• Catalog number
  4838-5
• Price
  Please ask
• Size
  5 ug

• Synonyms
  Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Phosphatase and tensin homolog, Mutated in Multiple advanced cancers 1, PTEN, MMAC1, TEP1, BZS, MHAM, PTEN1, 10q23del,
• Alternative_names
  Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1, BZS, MHAM, PTEN1, 10q23del,
• Description
  A tumour supressor protein
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Not available at this time
• Biological activity
  A Phosphatase specific activity of ≥ 400 nmol/min/mg was determined using the substrate phosphatidylinositol (3,4,5) triphosphate at a final concentration of 125 M in 20 mM Tris, pH 7.5, 150 mM NaCl, 50 M MgCl2, 0.05 % Tween-20, 0.2 % 2-mercaptoethanol.
• Molecular Weight
  80.0 kDa (GST tagged)
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Concentration
  100 µg/ml
• Appearance
  Liquid
• Physical form description
  100 µg/ml in 50 mM Tris-Acetate, pH 7.5, 150 mM NaCl, 0.1 mM EDTA, 10 mM glutathione, 25 mM DTT, 0.1 mM PMSF and 25% Glycerol.
• Background Information
  PTEN, a tumor suppressor, has been implicated in a large number of human tumors and is conserved from humans to worms. Characterization of PTEN protein showed that it is a phosphatase that acts on proteins and on 3-phosphorylated phosphoinositides, and can therefore modulate signal-transduction pathways that involve lipid second messengers. Recent results indicate that at least part of its role is to regulate the activity of the serine/threonine kinase AKT/PKB, and thus influence cell survival signaling. Recombinant PTEN is purified by proprietary chromatographic techniques.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PTEN  
• Gene symbol
  PTEN, PTENP1
• Short name
  PTEN, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  phosphatase and tensin homolog, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  phosphatase and tensin homolog, 10q23del and BZS and CWS1 and DEC and GLM2 and MHAM and MMAC1 and PTEN1 and TEP1, PTEN and IDBG-81409 and ENSG00000171862 and 5728, phosphatidylinositol-3, nuclei, Pten and IDBG-158878 and ENSMUSG00000013663 and 19211, PTEN and IDBG-637256 and ENSBTAG00000009498 and 540786

Gene info

• Identity
  HGNC:9588
• Gene
  PTEN
• Long gene name
  phosphatase and tensin homolog
• Synonyms gene
  • BZS
  • MHAM
• Synonyms
  • MMAC1
  • TEP1
  • PTEN1
• Synonyms name
  • mutated in multiple advanced cancers 1
• GenBank acession
  • U92436
• Locus
  10q23.31
• Discovery year
  1997-04-21
• Entrez gene record
  5728
• Pubmed identfication
  • 9090379
• RefSeq identity
  • NM_000314
• Classification
  • PTEN protein phosphatases
  • C2 tensin-type domain containing
  • Phosphoinositide phosphatases
• VEGA ID
  OTTHUMG00000018688
• Locus Specific Databases
  • LRG_311

Gene info

• Identity
  HGNC:9589
• Gene
  PTENP1
• Long gene name
  phosphatase and tensin homolog pseudogene 1
• Synonyms gene name
  • phosphatase and tensin homolog pseudogene 1 (functional)
• Synonyms
  • PTEN2
  • psiPTEN
  • PTH2
  • PTEN-rs
  • PTENpg1
• GenBank acession
  • AF023139
  • BC038293
  • BY797336
• Locus
  9p13.3
• Discovery year
  1999-09-09
• Entrez gene record
  11191
• Pubmed identfication
  • 9393738
  • 9620558
  • 20577206
• RefSeq identity
  • NR_023917
• VEGA ID
  OTTHUMG00000000410

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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