OxiSelect™ Cellular UV-Induced DNA Damage Staining test kit (CPD), Trial Size
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Catalog number
STA-327-T
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Price
Please ask
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Size
32 assays
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We send this reagant on
Room temperature
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The temperatureerature for storage is
4ºC
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Technical file
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SDS or safty data sheet
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Type of reagent
Oxidative Stress/Damage
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Related techniques
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, UV Induced DNA Damage test kits
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Use of the reagent
Our OxiSelect™ Cellular UV-Induced DNA Damage Staining test kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
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Cellular use
Measure CPD structures of DNA lesions induced by UV light
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Protocol information
Detect structures by immunofluorescence staining under a fluorescence microscope
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,68
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Description
Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription.
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Gene target
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Gene symbol
CPD, PRKDC, HLA-DOA, HOMER2
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Short name
OxiSelect™ Cellular UV- DNA Damage Staining test kit (CPD), Trial Size
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Technique
cellular, dna
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Alternative name
OxiSelect™ Cellular ultraviolet-Induced Desoxyribonucleic acid Damage coloring test reagent (CPD), Trial Size
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Alternative technique
tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cellular
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Gene info
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Identity
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Gene
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Long gene name
carboxypeptidase D
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-04-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
homer scaffold protein 2
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Synonyms gene name
- homer homolog 2 (Drosophila)
- homer scaffolding protein 2
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Synonyms
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GenBank acession
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Locus
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Discovery year
2003-01-28
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
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Tree numbers
- E01.370.225.812.735.830
- E05.200.812.735.830
- E05.478.566.380.810
- E05.478.566.639.810
- E05.478.594.760.830
- E05.601.470.380.810
- E05.601.470.639.810
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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