pLKO.1-HIF1A shRNA Plasmid

Quantity:

Kit

Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.

Gene target

pLKO 1-HIF1A shRNA
Gene symbol

HIF1A-AS3, HIF1A-AS1, HIF1A-AS2, HIF1A, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1
Short name

pLKO 1-HIF1A shRNA Plasmid
Technique

plasmid, plasmids in 1
Alternative name

pLKO.1-hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) short hairpin RNA Plasmid
Alternative technique

plasmids
Alternative to gene target

hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), bHLHe78 and HIF-1A and HIF-1alpha and HIF1 and HIF1-ALPHA and MOP1 and PASD8, HIF1A and IDBG-8510 and ENSG00000100644 and 3091, Hsp90 protein binding, nuclei, Hif1a and IDBG-148620 and ENSMUSG00000021109 and 15251, HIF1A and IDBG-646757 and ENSBTAG00000020935 and 281814

Quantity:

Identity

HGNC:4910
Gene

HIF1A
Long gene name

hypoxia inducible factor 1 subunit alpha
Synonyms gene name
- hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Synonyms
- MOP1
- HIF-1alpha
- PASD8
- HIF1
- bHLHe78
Synonyms name
- PAS domain-containing protein 8”, “Class E basic helix-loop-helix protein 78”, “Member of PAS protein 1
GenBank acession
- U22431
Locus

14q23.2
Discovery year

1995-03-27
Entrez gene record

3091
Pubmed identfication
- 8786149
- 9079689
RefSeq identity
- NM_001530
Classification
- Basic helix-loop-helix proteins
VEGA ID

OTTHUMG00000140344

Identity

HGNC:5670
Gene

IGHVII-1-1
Long gene name

immunoglobulin heavy variable (II)-1-1 (pseudogene)
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
Synonyms
- IGHV(II)-1-1
GenBank acession
- AB019441
Locus

14q32.33
Discovery year

2000-04-17
Entrez gene record

28377
RefSeq identity
- NG_001019
Classification
- Immunoglobulin heavy locus at 14q32.33
VEGA ID

OTTHUMG00000152325

Identity

HGNC:5760
Gene

IGKV1OR2-1
Long gene name

immunoglobulin kappa variable 1/OR2-1 (pseudogene)
Synonyms gene
- IGKVPZ1
- IGKV1OR-1
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
Synonyms
- IGKV1/OR-1
- IGKV1/OR2-1
GenBank acession
- Z12367
Locus

2p11.1
Discovery year

2000-04-18
Entrez gene record

3531
Pubmed identfication
- 2853098
Classification
- Immunoglobulin kappa (IGK) orphons
VEGA ID

OTTHUMG00000155081