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Description
Sensitive, Colorimetric Assay
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Summary
• Detection method- Absorbance (450 nm) • Species reactivity-human • Application- Quantitative measurement of native and recombinant p53.
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Detection Method
Absorbance (450 nm)
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Species Reactivity
human
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Applications
This ELISA kit is used for quantitative measurement of native and recombinant p53
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Sample Type
• Cell lysate and cell culture supernatant • Serum and plasma
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Features Benefits
• Easy, convenient and time-saving method to assay for human p53. • Detection Range: 0.2 ng/ml to 25.6 ng/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.
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Storage Conditions
MT
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
BioVision’s p53 Enzyme-Linked Immunosorbent Assay (ELISA) Kit is an in vitro assay for the quantitative measurement of native and recombinant human p53. The p53 protein is encoded by the TP53 gene. It is composed of 393 amino acids but its apparent molecular mass is 53 kDa. p53 binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. It acts as a pivotal suppressor of inappropriate cell proliferation. By initiating suppressive effects through induction of apoptosis, cell senescence, or transient cell-cycle arrest, p53 plays an important role in cancer suppression, developmental regulation, and aging. p53 is found in increased amounts in a wide variety of transformed and tumor cells, where its concentration is increased 5-1000 fold over the concentration in normal cells. Meanwhile, p53 is also frequently mutated or inactivated in about 60% of cancers. The p53 ELISA assay employs an antibody specific for human p53 coated on a 96-well plate. Standards and samples are pipetted into wells and p53 present in the sample is bound to the wells by immobilized antibody. Wells are washed and a biotin-labeled human p53 specific detection antibody is added. After washing away unbound detection antibody, a streptavidin HRP-conjugate is added to the wells. The wells are again washed, a TMB substrate solution is added, and color develops in proportion to the amount of bound p53. Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Sensitivity of the kit is 0.1 ng/ml and detection range is from 0.2 ng/ml to 25.6 ng/ml. Recovery within this range is 86.3%. The intra-assay reproducibility as measured by the coefficient of variation (CV) is < 8 % & inter-assay has CV < 12 %.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
MDM4, TP53, LNCPRESS2
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Short name
p53 ( ) ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
p53 (H. sapiens) Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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