Recombinant Human Granulin / GRN / Progranulin Protein (His tag) 100µg
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Catalog numberPKSH031405-100µg
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PricePlease ask
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Size100µg
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SynonymCLN11;GEP;GP88;Granulin;PCDGF;PEPI;PGRN;Progranulin
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ActivityNA
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SequenceMet 1-Leu 593
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Fusion tagC-His
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AccessionNP_002078.1
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Expressed HostHEK293 Cells
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ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity>95 % as determined by SDS-PAGE
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Endotoxin<1.0 EU per µg as determined by the LAL method
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass63.2 kDa
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AP Mol Mass100-105 kDa
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FormulationLyophilized from sterile PBS, pH 7.4
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ReconstitutionPlease refer to it for detailed information.
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BackgroundGranulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.
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Conjugationhistidine
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolGRN
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Short nameRecombinant Granulin / GRN / Progranulin Protein (His tag) 100µg
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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LabelHis
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens Granulin / granulin / Progranulin Protein (histidine detection labelled) 100µg
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Alternative techniquerec
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Alternative to gene targetgranulin, CLN11 and GEP and GP88 and PCDGF and PEPI and PGRN, GRN and IDBG-53782 and ENSG00000030582 and 2896, poly(A) RNA binding, Extracellular, Grn and IDBG-212642 and ENSMUSG00000034708 and 14824, GRN and IDBG-640956 and ENSBTAG00000018823 and 767942
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Gene info
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Identity
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Gene
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Long gene namegranulin precursor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-11-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data