Recombinant Mouse Granulin / GRN / Progranulin Protein (His tag)

• Catalog number
  PKSM040703-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  epithelin;Pgrn
• Activity
  NA
• Sequence
  Met 1-Leu 589
• Fusion tag
  C-His
• Accession
  NP_032201.2
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 90 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  63 kDa
• AP Mol Mass
  70-90 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Granulin   GRN   Progranulin   Protein   His   tag  
• Gene symbol
  GRN
• Short name
  Recombinant Mouse Granulin / GRN / Progranulin Protein (His tag)
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse Granulin / granulin / Progranulin Protein (histidine detection labelled)
• Alternative technique
  rec, murine
• Alternative to gene target
  granulin, CLN11 and GEP and GP88 and PCDGF and PEPI and PGRN, GRN and IDBG-53782 and ENSG00000030582 and 2896, poly(A) RNA binding, Extracellular, Grn and IDBG-212642 and ENSMUSG00000034708 and 14824, GRN and IDBG-640956 and ENSBTAG00000018823 and 767942

Gene info

• Identity
  HGNC:4601
• Gene
  GRN
• Long gene name
  granulin precursor
• Synonyms
  • PCDGF
  • PGRN
  • CLN11
• Synonyms name
  • progranulin
• GenBank acession
  • M75161
• Locus
  17q21.31
• Discovery year
  1992-11-30
• Entrez gene record
  2896
• Pubmed identfication
  • 1417868
  • 9826678
• RefSeq identity
  • NM_002087
• VEGA ID
  OTTHUMG00000181816
• Locus Specific Databases
  • LRG_661

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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