Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit (Colorimetric)
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Catalog number
K225-100
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Price
Please ask
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Size
100 assays
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Description
Industry's first kit to screen/study/characterize potential inhibitors of G6PDH
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Summary
• Detection method - Absorbance (OD = 450 nm). • Applications-Screening/characterizing/studying potential inhibitors of Glucose-6-Phosphate Dehydrogenase
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Detection Method
Absorbance (OD = 450 nm).
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Species Reactivity
N/A
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Applications
Screening/characterizing/studying potential inhibitors of Glucose-6-Phosphate Dehydrogenase
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Sample Type
Small molecules
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Features Benefits
Simple and sensitive assay. Fast protocol - can be perforemed in 30 min. HTP adaptable.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme which plays a key role in the Pentose Phosphate Pathway (PPP). The PPP pathway supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Recently studies have found increased G6PDH activity in various human tumors and knockdown of this G6PDH decreases cell proliferation and enhances apoptosis in vitro. Therefore, targeting this key enzyme is essential for developing novel therapeutic approach for treatment/cure of cancer. In BioVision’s G6PDH assay kit, Glucose-6-Phosphate is oxidized by glucose-6-phosphate dehydrogenase to generate 6-Phosphoglucono-δ-Lactone and form NADH, which reacts with a probe to produce a signal at OD 450 nm. In the presence of G6PDH inhibitor, these reactions are impeded, thus decreasing the rate and/or extent of generation of G6PDH-dependent absorbance. Thus, this kit provides a sensitive, quick, and easy method for screening potential inhibitors of G6PDH. G6PDH Inhibitor Control is included to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed in less than 30 min.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbol
MIR1302-6, PIRC24, PIRC28, PIRC26, ADCP1, PIRC27, PIRC25
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Short name
Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit (Colorimetric)
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Technique
colorimetric, colorimetry
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Alternative name
Glucose-6-Phosphate Dehydrogenase suppressor detection reagent (colorimetry)
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Alternative technique
assays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
microRNA 1302-6
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Product images
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