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Description
A quick and sensitive way for screening, studying and characterizing the potential inhibitors of ALDH3A1
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Summary
Aldehyde Dehydrogenases (ALDHs; EC 1.2.1.3) are oxidizing enzymes that play an important, anti-oxidative role by oxidizing aldehydes generated from the metabolism of amino acids, fatty acids, food, xenobiotic drugs etc. to carboxylic acids. Aldehyde Dehydrogenase 3A1 (ALDH3A1), a member of the ALDH superfamily is highly expressed in various cancers such as hepatoma, lung adenocarcinoma, myeloma, breast cancer etc. It has been shown to play an important role in the survival, metastasis, and chemo-resistance of cancer cells. Thus, the selective inhibition of ALDH3A1 in cancer cells could restore chemosensitivity thereby allowing lower effective dosages and reducing unwanted side effects. BioVision’s ALDH3A1 Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing the potential inhibitors of ALDH3A1. In this assay, ALDH3A1 oxidizes a specific substrate and generates NADH as one of the intermediate. NADH then couples with the PicoProbe™ to form a stable fluorophore, which is measured at Ex/Em = 535/587 nm. ALDH3A1 Inhibitor Control is included in the kit to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed within 30-60 min.
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Species Reactivity
Human
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Applications
Screening and characterizing potential ALDH3A1 inhibitors.
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Features Benefits
ALDH3A1 Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing the potential inhibitors of ALDH3A1.,The assay is high-throughput adaptable and can be performed within 30-60 min.
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Storage Conditions
-20°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
Aldehyde Dehydrogenases (ALDHs; EC 1.2.1.3) are oxidizing enzymes that play an important, anti-oxidative role by oxidizing aldehydes generated from the metabolism of amino acids, fatty acids, food, xenobiotic drugs etc. to carboxylic acids. Aldehyde Dehydrogenase 3A1 (ALDH3A1), a member of the ALDH superfamily is highly expressed in various cancers such as hepatoma, lung adenocarcinoma, myeloma, breast cancer etc. It has been shown to play an important role in the survival, metastasis, and chemo-resistance of cancer cells. Thus, the selective inhibition of ALDH3A1 in cancer cells could restore chemosensitivity thereby allowing lower effective dosages and reducing unwanted side effects. BioVision’s ALDH3A1 Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing the potential inhibitors of ALDH3A1. In this assay, ALDH3A1 oxidizes a specific substrate and generates NADH as one of the intermediate. NADH then couples with the PicoProbe™ to form a stable fluorophore, which is measured at Ex/Em = 535/587 nm. ALDH3A1 Inhibitor Control is included in the kit to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed within 30-60 min.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
