Mouse Anti-Mouse Protocadherin Gamma A3 Monoclonal IgG1, Clone S144-17
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Catalog numberSMC-451D-A633
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PricePlease ask
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Size100 µg
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CloneS144-17
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ImmunogenFusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-A3. Human: 80% identity (68/84 amino acids identical) >75% identity with other Gamma-protocadherin-A protein.
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Antibody s full descriptionMouse Anti-Mouse Protocadherin Gamma A3 Monoclonal IgG1 Antibody, Clone: S144-17: ATTO 633
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Antibody s categoryMonoclonal Antibodies
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Antibody s other namePCDHGA3 Antibody, PCDG3 Antibody, PCDH gamma A3 Antibody, Protocadherin gamma subfamily A 3 Antibody, Protocadherin gamma-A3 Antibody, Gamma Protocadherin-A3 Antibody, Gamma-Protocadherin-A3 Antibody
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Raised inMouse
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Antibody s targetProtocadherin Gamma A3
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Primary research fieldsNeuroscience, Cell Structure, Membrane Markers
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman, Mouse
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~100kDa. No cross-reactivity against other Protocadherin Gamma -A, -B or -C proteins.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-451 was sufficient for detection of Protocadherin Gamma A3 in 20 µg of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma A3) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextProtocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin (PCDH) gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDHGA3 (Protocadherin gamma-A3) is a 932 amino acid that is one of 22 proteins encoded by the protocadherin gamma cluster. The protocadherein gamma cluster consists of three subfamilies (A, B and C) and PCDHGA3 is a member of the gamma subfamily A. PCDHGA3 is a type I transmembrane receptor containing six cadherin motifs and is expressed in the central nervous system where it localizes to synapses. Members of the gamma cluster of protocadherins are essential for neuronal survival. There are two isoforms of PCDHGA3 that are produced as a result of alternative splicing events.
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Released date20-Nov-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_291064.1
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Gene number93711
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Protein numberQ91XY5
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (SMC-451). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (SMC-451) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma A3 Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A3 protein using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (SMC-451). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (SMC-451) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-Protocadherin Gamma A3 Antibody [S144-17] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-451) Mouse Anti-Protocadherin Gamma A3 Antibody [S144-17] used in Western Blot (WB) on Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 (SMC-451)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolPIRC95, PIRC91, PIRC90, SNORD115-17, IGHV3OR16-17, PIRC92, PIRC93, SNORD116-17
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Short nameMouse Anti-Mouse Protocadherin Gamma A3 Monoclonal IgG1, Clone S144-17
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelATTO 633
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Protocadherin g A3 monoclonal IgG1, clonality S144-17
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Alternative techniquemurine, antibodies
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Clone nameS144-17
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Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 95
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 91
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 90
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-17
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3/OR16-17 (non-functional)
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Synonyms
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Locus
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Discovery year2020-06-26
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 92
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 93
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-17
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
