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Mouse Anti-Mouse Protocadherin Gamma A3 Monoclonal IgG1, Clone S144-17

  • Catalog number
    SMC-451D-APC
  • Product name
    Mouse Anti-Mouse Protocadherin Gamma A3 Monoclonal IgG1, Clone S144-17
  • Size
    100 µg
  • Label
    APC
  • Clone
    S144-17
  • Immunogen
    Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma- protocadherin-A3. Human: 80% identity (68/84 amino acids identical) >75% identity with other Gamma-protocadherin-A protein.
  • Antibody s full description
    Mouse Anti-Mouse Protocadherin Gamma A3 Monoclonal IgG1 Antibody, Clone: S144-17: APC
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    PCDHGA3 Antibody, PCDG3 Antibody, PCDH gamma A3 Antibody, Protocadherin gamma subfamily A 3 Antibody, Protocadherin gamma-A3 Antibody, Gamma Protocadherin-A3 Antibody, Gamma-Protocadherin-A3 Antibody
  • Raised in
    Mouse
  • Isotype
    IgG1, IgG1
  • Antibody s target
    Protocadherin Gamma A3
  • Primary research fields
    Neuroscience, Cell Structure, Membrane Markers
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF
  • Antibody s reactivity
    Human, Mouse
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~100kDa. No cross-reactivity against other Protocadherin Gamma -A, -B or -C proteins.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-451 was sufficient for detection of Protocadherin Gamma A3 in 20 µg of COS cell lysate (transiently transfected with GFP-tagged Protocadherin Gamma A3) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cell Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin (PCDH) gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDHGA3 (Protocadherin gamma-A3) is a 932 amino acid that is one of 22 proteins encoded by the protocadherin gamma cluster. The protocadherein gamma cluster consists of three subfamilies (A, B and C) and PCDHGA3 is a member of the gamma subfamily A. PCDHGA3 is a type I transmembrane receptor containing six cadherin motifs and is expressed in the central nervous system where it localizes to synapses. Members of the gamma cluster of protocadherins are essential for neuronal survival. There are two isoforms of PCDHGA3 that are produced as a result of alternative splicing events.
  • Released date
    20-Nov-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_291064.1
  • Gene number
    93711
  • Protein number
    Q91XY5
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (SMC-451). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (SMC-451) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Protocadherin Gamma A3 Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 showing detection of ~100 kDa Protocadherin Gamma A3 protein using Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody, Clone S144-17 (SMC-451). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Protocadherin Gamma A3 Monoclonal Antibody (SMC-451) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~100 kDa. Mouse Anti-Protocadherin Gamma A3 Antibody [S144-17] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-451) Mouse Anti-Protocadherin Gamma A3 Antibody [S144-17] used in Western Blot (WB) on Monkey COS cells transfected with GFP-tagged Gamma-protocadherin-A3 (SMC-451)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Protocadherin Gamma A3 Monoclonal IgG1 S144-17
    • Gene info
    • Identity:HGNC:37599
    • Gene:PIRC95
    • Long gene name:piwi-interacting RNA cluster 95
    • Discovery year:2009-11-05
    • Entrez gene record:100313935
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37595
    • Gene:PIRC91
    • Long gene name:piwi-interacting RNA cluster 91
    • Discovery year:2009-11-05
    • Entrez gene record:100313900
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37594
    • Gene:PIRC90
    • Long gene name:piwi-interacting RNA cluster 90
    • Discovery year:2009-11-05
    • Entrez gene record:100313798
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    Gene info
    • Identity:HGNC:55105
    • Gene:IGHV3OR16-17
    • Long gene name:immunoglobulin heavy variable 3/OR16-17 (non-functional)
    • Synonyms:IGHV3/OR16-17
    • Discovery year:2020-06-26
    • Entrez gene record:390714
    • Classification:Immunoglobulin heavy (IGH) orphons
    Gene info
    • Identity:HGNC:37596
    • Gene:PIRC92
    • Long gene name:piwi-interacting RNA cluster 92
    • Discovery year:2009-11-05
    • Entrez gene record:100313920
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
    • Identity:HGNC:37597
    • Gene:PIRC93
    • Long gene name:piwi-interacting RNA cluster 93
    • Discovery year:2009-11-05
    • Entrez gene record:100313934
    • Pubmed identification:17881367
    • Classification:Piwi-interacting RNA clusters
    Gene info
  • Gene symbol
    PIRC95, PIRC91, PIRC90, SNORD115-17, IGHV3OR16-17, PIRC92, PIRC93, SNORD116-17
  • Host
    mouse
  • isotype filter
    • IgG1
  • Short name
    Mouse Anti-Mouse Protocadherin Gamma A3 Monoclonal IgG1, Clone S144-17
  • label filter
    • APC
  • Clone name
    S144-17
  • technique filter
    • anti-
    • Mouse
    • anti
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Alternative technique
    murine, antibodies