Human Interleukin-13 Recombinant

• Background: Mouse IL-13 is produced primarily by activated Th2 cells. Human IL-13 is expressed in activated T- helper cells (resembling Th0, Th1, Th2), and T-cells expressing CD8. The alpha chain of the IL1-3 receptor has weak binding activity for IL-13. High affinity receptors are formed when the receptor alpha chains of IL-13 and IL-4 receptors are coexpressed. The receptors for IL-13 and IL-4 share a common gamma subunit that is found also in the IL-2 receptor. Different IL-13 receptor structures have been shown to exist on various cell types and the IL-13 receptor may share more than one component with IL-4 receptor. IL-13 down-modulates macrophage activity, reducing the production of pro-inflammatory cytokines and chemokines in response to IFN-gamma or bacterial lypopolysaccharides. IL-13 enhances the production of the IL-1 receptor antagonist IL-1ra. IL-13 also decreases the production of nitric oxide by activated macrophages, leading to a decrease in parasiticidal activity. IL-13 induces differentiation of human monocytes, enhances survival time in culture, and also induces differentiation and proliferation and Isotype switching in B-cells.
• Description: Recombinant IL-13 is a disulfide-linked monomer protein consisting of 115 amino acid residues, and migrates as an approximately 13 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-13 mature chain was expressed inE. coli.
• UniProt: P35225
• Endotoxin: < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
• Purity: ≥ 95% by SDS-PAGE and HPLC
• Format: Lyophilized protein
• Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
• Shipping Conditions: Gel Packs or Dry Ice
• Storage Conditions: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
• Calculated Molecular Weight: 13 kDa
• Formulation: Lyophilized from 0.2 µm filtered concentrated (1 mg/ml) solution in PBS, pH 7.2.