Fibronectin-1, Human Recombinant

• Background:

  Recombinant human FN is a major component of the provisional ECM produced during sprouting angiogenesis.

• Description:

  Human fibronectin 1 (FN1, also known as CD36), Genbank Accession number X02761.1, a.a. 2175 - 2386 with a C-terminal His-tag, expressed in an HEK293 cell expression system. MW =28 kDa. This protein migrates at a higher than expected molecular weight due to glycosylation.

• UniProt:

  P02751

• Tag:

  C-terminal His tag

• Endotoxin:

  Endotoxin level was found to be < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C

• Calculated Molecular Weight:

  28 kDa

• Formulation:

  Lyophilized from from 0.2 μm filtered PBS solution, pH7.2,5% Trehalose. at 0.1-1.0 mg/mL in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.