Phosphatidylserine Assay Kit (Fluorometric)

• Catalog number
  K565-100
• Price
  Please ask
• Size
  100 assays

• Description
  The first kit in the market to measure Phosphatidylserine in samples
• Summary
  • Detection method- Fluorescence (Ex/Em= 535/587 nm) • Sample type- cell and/or tissue lipid extracts • Species reactivity- Mammalian • Applications- This assay provides a convenient and non-ELISA alternative to detect Phosphatidylserine amount in biological samples.
• Detection Method
  Fluorescence (Ex/Em= 535/587 nm)
• Species Reactivity
  Eukaryotes
• Applications
  This assay provides a convenient and non-ELISA alternative to detect Phosphatidylserine amount in biological samples.
• Sample Type
  Cell and Tissue lipid extract
• Features Benefits
  Simple, fast, does not require lenghty incubation times
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Phosphatidylserine (PS) is a glycerophospholipid consisting of a phosphatidyl group attached to L-serine via a phosphodiester linkage. PS is a critical component of the cellular plasma membrane and accounts for 2-15% of plasma membrane lipid composition, depending on the cell/tissue type. The highest concentrations of PS are found in neuronal tissues, where it is critical for maintaining conduction velocity in myelinated neurons, as well as for higher order cognitive skills such as learning and memory. In normal, healthy cells, PS is held in the inner membrane surface (facing the cytosol) by the lipid transporter protein flippase. However, in apoptotic cells, PS molecules ‘shuffle’ between the inner and outer plasma membrane monolayers. When PS molecules flip to the extracellular (outer) surface of the cell membrane, they act as a signal for macrophages to engulf and digest the (apoptotic) cell. BioVision’s Phosphatidylserine Assay Kit allows for quantification of PS in lipid extracts of cell/tissue lysates. The assay is based on the enzymatic cleavage of PS to yield phosphatidic acid and L-serine, which is subsequently metabolized and reacts with a probe to form a stable fluorophore (Ex/Em = 538/587 nm). The assay is selective for PS (other phospholipids such as phosphatidylcholine, phosphatidylethanolamine or phosphatidic acid do not interfere). The kit is high-throughput ready and highly sensitive (can detect as little as 50 pmole/well of PS (5 μM in a 10 μl sample volume)).
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Phosphatidylserine   Kit   Fluorometric  
• Short name
  Phosphatidylserine Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Phosphatidylserine test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products