Genomic PCR Grade Proteinase K Powder
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Catalog number
506-PKP -0200
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Price
Please ask
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Size
200mg
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Availability
Please contact us to check the latest availability and to check prices. For larger or bulk quantity a discount may apply
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CAS No
39450-01-6
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Application
nonspecific serine protease
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Ordering
Product made-to-order. Please contact us for latest inventory stock. Delivery is 1-2 weeks upon order confirmation
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Package weight
The standard package weight with individual packaging is 0,05
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Before use
Store at 2-8°C
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Shipped to you
Shipped at room temperature
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Technical file
Please inquire for the latest datasheet, certificate of analysis and SDS
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Precautions
For Research Use Only. Manufactured in USA
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene
Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
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Gene target
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Short name
Genomic PCR Grade Proteinase K Powder
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Technique
PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Alternative name
Genomic PCR test kit Grade Proteinase K Powder
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Alternative technique
dna-amplification
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MeSH Data
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Name
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Concept
Scope note:
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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