Mouse Anti-Mouse Versican Monoclonal IgG1, Clone S351-23
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Catalog numberSMC-439D
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Price:
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Size100 µg
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CloneS351-23
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ImmunogenFusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
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Antibody s full descriptionMouse Anti-Mouse Versican Monoclonal IgG1 Antibody, Clone: S351-23: Unconjugated
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameChondroitin sulfate proteoglycan 2 Antibody, CSPG2 Antibody, ERVR Antibody, GHAP Antibody, PG-M Antibody, VCAN Antibody, Chondroitin sulfate proteoglycan 2 Antibody, Chondroitin sulfate proteoglycan core protein 2 Antibody, Glial hyaluronate binding protein Antibody, Glial hyaluronate-binding protein Antibody, Large fibroblast proteoglycan Antibody, Large fibroblast proteoglycan Antibody, PGM Antibody, V1 Neo Antibody, Versican core protein Antibody, Versican proteoglycan Antibody, Versican V0 Antibody, WGN 1 Antibody, WGN Antibody, WGN1 Antibody
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Raised inMouse
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Antibody s targetVersican
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Primary research fieldsNeuroscience, Cell Signaling, Scaffolds
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BrandnameNone
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Antibodies applicationsWB, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.1% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects >350kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-439 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellExtracellular Space , Extracellular Matrix
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextVersican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a Lectinlike sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length anddegree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in lesions of athero-sclerosis suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels.
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Bibliography1. Dours-Zimmermann M.T. and Zimmermann D.R. (1994) J. Biol. Chem. 52: 32992-32998. 2. Evanko S.P., Angello J.C. and Wight T.N. (1999) Arterioscler. Thromb. Vasc. Biol. 4:1004-1013. 3. Lemire J.M., et al. (1999) Arterioscler. Thromb. Vasc. Biol. 7: 1630-1639. 4.Wight T.N. (2002) Curr. Opin. Cell Biol. 5: 617-623. 5. Touab M., Villena J., Barranco C., Arumi-Uria M. and Bassols A. (2002) Am. J. Pathol. 2: 549-557.
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Released date1-Mar-2013
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberAAH96495
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Gene number13003
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Protein numberQ62059
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (SMC-439). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (SMC-439) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Versican Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versican protein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (SMC-439). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (SMC-439) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands. Mouse Anti-Versican Antibody [S351-23] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-439) Mouse Anti-Versican Antibody [S351-23] used in Western Blot (WB) on Rat Brain Membrane and brain (SMC-439)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGHV3-23, IGKV2D-23, SNORD116-23, SNORD115-23, SNORD114-23, IGKV2-23, ERVW-23, IGLV2-23, CEMP1, ERVK-23
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Short nameMouse Anti-Mouse Versican Monoclonal IgG1, Clone S351-23
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelUnconjugated
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Versican monoclonal IgG1, clonality S351-23
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Alternative techniquemurine, antibodies
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Clone nameS351-23
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-23
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2D-23 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2D-23
- immunoglobulin kappa variable 2D-23 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-23
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-23
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-23
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2-23 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2-23
- immunoglobulin kappa variable 2-23 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group W member 23
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Synonyms gene name
- endogenous retrovirus group W, member 23
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GenBank acession
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Locus
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Discovery year2013-10-21
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 2-23
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecementum protein 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-09-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group K member 23
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Synonyms gene name
- endogenous retrovirus group K, member 23
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Synonyms
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GenBank acession
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Locus
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Discovery year2011-05-05
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Pubmed identfication
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data