Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
Antibody s full description
Mouse Anti-Mouse Versican Monoclonal IgG1 Antibody, Clone: S351-23: ATTO 700
Antibody s category
Antibody s other name
Chondroitin sulfate proteoglycan 2 Antibody, CSPG2 Antibody, ERVR Antibody, GHAP Antibody, PG-M Antibody, VCAN Antibody, Chondroitin sulfate proteoglycan 2 Antibody, Chondroitin sulfate proteoglycan core protein 2 Antibody, Glial hyaluronate binding protein Antibody, Glial hyaluronate-binding protein Antibody, Large fibroblast proteoglycan Antibody, Large fibroblast proteoglycan Antibody, PGM Antibody, V1 Neo Antibody, Versican core protein Antibody, Versican proteoglycan Antibody, Versican V0 Antibody, WGN 1 Antibody, WGN Antibody, WGN1 Antibody
Antibody s target
Primary research fields
Neuroscience, Cell Signaling, Scaffolds
Antibody s reactivity
Human, Mouse, Rat
Antibody s dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Protein G Purified
Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Antibody s concentration
Antibody s specificity
Blue Ice or 4ºC
Antibody certificate of analysis
1 µg/ml of SMC-439 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell
Extracellular Space , Extracellular Matrix
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Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a Lectinlike sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length anddegree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in lesions of athero-sclerosis suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels.
1. Dours-Zimmermann M.T. and Zimmermann D.R. (1994) J. Biol. Chem. 52: 32992-32998. 2. Evanko S.P., Angello J.C. and Wight T.N. (1999) Arterioscler. Thromb. Vasc. Biol. 4:1004-1013. 3. Lemire J.M., et al. (1999) Arterioscler. Thromb. Vasc. Biol. 7: 1630-1639. 4.Wight T.N. (2002) Curr. Opin. Cell Biol. 5: 617-623. 5. Touab M., Villena J., Barranco C., Arumi-Uria M. and Bassols A. (2002) Am. J. Pathol. 2: 549-557.
To be tested
To be tested
Antibody s datasheet
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Representative figure link
Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (SMC-439). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (SMC-439) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Versican Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versican protein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (SMC-439). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (SMC-439) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands. Mouse Anti-Versican Antibody [S351-23] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-439) Mouse Anti-Versican Antibody [S351-23] used in Western Blot (WB) on Rat Brain Membrane and brain (SMC-439)
Country of production
Total weight kg
Net weight g
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.