Mouse Anti-Mouse Versican Monoclonal IgG1, Clone S351-23

  • Clone
    S351-23
  • Immunogen
    Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein
  • Antibody s full description
    Mouse Anti-Mouse Versican Monoclonal IgG1 Antibody, Clone: S351-23: ATTO 700
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Chondroitin sulfate proteoglycan 2 Antibody, CSPG2 Antibody, ERVR Antibody, GHAP Antibody, PG-M Antibody, VCAN Antibody, Chondroitin sulfate proteoglycan 2 Antibody, Chondroitin sulfate proteoglycan core protein 2 Antibody, Glial hyaluronate binding protein Antibody, Glial hyaluronate-binding protein Antibody, Large fibroblast proteoglycan Antibody, Large fibroblast proteoglycan Antibody, PGM Antibody, V1 Neo Antibody, Versican core protein Antibody, Versican proteoglycan Antibody, Versican V0 Antibody, WGN 1 Antibody, WGN Antibody, WGN1 Antibody
  • Raised in
    Mouse
  • Antibody s target
    Versican
  • Primary research fields
    Neuroscience, Cell Signaling, Scaffolds
  • Brandname
    None
  • Antibodies applications
    WB, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects >350kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-439 was sufficient for detection of Versican in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Extracellular Space , Extracellular Matrix
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Versican (chondroitin sulfate proteoglycan 2) is a large extracellular matrix proteoglycan involved in cell growth and differentiation. Important as a structural molecule, versican creates loose and hydrated matrices during key events in development and disease. The protein contains hyaluronic acid and glycosminoglycan-binding domains, epidermal growth factor-like repeats, a Lectinlike sequence and a complement regulatory protein-like domain. Splice variants differ greatly in length anddegree of modification by glycoaminoglycan chains. Accumulation around smooth muscle cells in lesions of athero-sclerosis suggests a role for versican in atherogenesis. Versican, differentially expressed in human melanoma, plays a role in tumor development and may be a reliable marker for clinical diagnosis. The organization of HA- and versican-rich pericellular matrices may faciliatate migration and mitosis by diminishing cell surface adhesivity and affecting cell shape through steric exclusion and the viscous properties of HA proteoglycan gels.
  • Bibliography
    1. Dours-Zimmermann M.T. and Zimmermann D.R. (1994) J. Biol. Chem. 52: 32992-32998. 2. Evanko S.P., Angello J.C. and Wight T.N. (1999) Arterioscler. Thromb. Vasc. Biol. 4:1004-1013. 3. Lemire J.M., et al. (1999) Arterioscler. Thromb. Vasc. Biol. 7: 1630-1639. 4.Wight T.N. (2002) Curr. Opin. Cell Biol. 5: 617-623. 5. Touab M., Villena J., Barranco C., Arumi-Uria M. and Bassols A. (2002) Am. J. Pathol. 2: 549-557.
  • Released date
    1-Mar-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    AAH96495
  • Gene number
    13003
  • Protein number
    Q62059
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (SMC-439). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (SMC-439) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Versican Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane and brain showing detection of 350kDa Versican protein using Mouse Anti-Versican Monoclonal Antibody, Clone S351-23 (SMC-439). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane and brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Versican Monoclonal Antibody (SMC-439) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands. Mouse Anti-Versican Antibody [S351-23] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-439) Mouse Anti-Versican Antibody [S351-23] used in Western Blot (WB) on Rat Brain Membrane and brain (SMC-439)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    IGHV3-23, IGKV2D-23, SNORD116-23, SNORD115-23, SNORD114-23, IGKV2-23, ERVW-23, IGLV2-23, CEMP1, ERVK-23
  • Short name
    Mouse Anti-Mouse Versican Monoclonal IgG1, Clone S351-23
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    ATTO 700
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toMouse Versican monoclonal IgG1, clonality S351-23
  • Alternative technique
    murine, antibodies
  • Clone name
    S351-23
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 2D-23 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 2D-23
    • immunoglobulin kappa variable 2D-23 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin kappa locus at 2p11.2
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 2-23 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 2-23
    • immunoglobulin kappa variable 2-23 pseudogene
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin kappa locus at 2p11.2
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    endogenous retrovirus group W member 23
  • Synonyms gene name
    • endogenous retrovirus group W, member 23
  • GenBank acession
  • Locus
  • Discovery year
    2013-10-21
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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Mouse Anti Mouse Versican Monoclonal IgG1 Clone S351 23
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