Mouse Anti-Mouse Cav3.1 Monoclonal IgG1, Clone S178A-9
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Catalog numberSMC-405D-STR
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PricePlease ask
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Size100 µg
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CloneS178A-9
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ImmunogenFusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1
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Antibody s full descriptionMouse Anti-Mouse Cav3.1 Monoclonal IgG1 Antibody, Clone: S178A-9: Streptavidin
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameCACNA1G Antibody, CaV T1 Antibody, Cav3 1 Antibody, cav3 1c Antibody, KIAA1123 Antibody, MGC117234 Antibody, NBR13 Antibody, voltage gated calcium channel cav3.1 Antibody, calcium channel voltage dependent alpha 1G subunit Antibody, calcium channel voltage dependent T type alpha 1G subunit Antibody, calcium channel voltage dependent T type alpha1G subunit Antibody, voltage dependent calcium channel alpha 1G subunit isoform 11 Antibody, voltage dependent T type calcium channel subunit alpha 1G Antibody, Voltage gated calcium channel subunit alpha Cav3 1 Antibody
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Raised inMouse
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Antibody s targetCav3.1
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Primary research fieldsNeuroscience, Ion Channels, Calcium Channels, Voltage-Gated Calcium Channels
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BrandnameNone
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Antibodies applicationsWB, IHC, ICC/IF
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), ICC/IF (1:100);optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis1 µg/ml of SMC-405 was sufficient for detection of Cav3.1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellMembrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextCalcium channel CaV3.1 (a1G) is a low-voltage-activated T-type calcium channel. Such T-type channels are expressed throughout the body. In the heart, they may be involved in pacemaker current. In neurons, these channels may play a secondary pacemaker role (1). With the ubiquitous expression, it is not surprising that alterations in channel function have been implicated in disease. Drugs that act to block T-type calcium channels are used as anti-hypertensives, antiepileptic's, and blocking of T-type calcium channels may be involved in the action of some anesthetics and antipsychotics as well (1). Much remains to be determined about the precise cellular localization, in vivo physiological roles, roles in disease states and possible routes to modulate their structure/function to ameliorate effects of disease.
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Bibliography1. Perez-Reyes E. (2003). Physiol. Rev., 83: 117-161.
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Released date16-May-2012
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_001106284.1
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Gene number12291
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Protein numberQ9WUT2
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.1 Monoclonal Antibody, Clone S178A-9 (SMC-405). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody (SMC-405) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. Western Blot analysis of Rat brain membrane lysate showing detection of Cav3.1 Calcium Channel protein using Mouse Anti-Cav3.1 Calcium Channel Monoclonal Antibody, Clone S178A-9 (SMC-405). Primary Antibody: Mouse Anti-Cav3.1 Calcium Channel Monoclonal Antibody (SMC-405) at 1:1000. Mouse Anti-Cav3.1 Antibody [S178A-9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-405) Mouse Anti-Cav3.1 Calcium Channel Antibody [S178A-9] used in Western Blot (WB) on Rat brain membrane lysate (SMC-405)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolRNU6-9, PIRC47, PIRC38, PIRC43, PIRC45, PIRC39, PIRC44
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Short nameMouse Anti-Mouse Cav3 1 Monoclonal IgG1, Clone S178A-9
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG1, IgG1
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LabelStreptavidin
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SpeciesMouse, Mouses
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Alternative nameMouse Antibody toMouse Cav3.1 monoclonal IgG1, clonality S178A-9
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Alternative techniquemurine, antibodies
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Clone nameS178A-9
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Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 9
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Synonyms
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Locus
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Discovery year2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 47
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 38
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 43
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 45
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 39
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 44
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
