FITC-Conjugated Rat IgG1 Isotype Control

• Catalog number
  RICIGG1-02MG
• Price
  Please ask
• Size
  200 µg

• Clone
  RTK2071
• Conjugated
  FITC
• Tested application
  Flow Cytometry
• Long description
  This FITC-Conjugated Rat IgG1 Isotype Control was affinity purified from rabbit serum. It does not cross-react with any human or animal antigen
• Storage buffer
  PBS, 0,09% sodium Azide, may contain carrier protein-stabilizer
• Shipping conditions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Technical datasheet
  Please contact us to receive the latest datasheet, the certificate of analysis or the MSDS
• Regulation status
  This antibody is intended for Research Use Only.
• Other products
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• Properties
  This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Conjugation
  Anti-FITC Antibody
• Group
  isotypes
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  Conjugated   IgG1  
• Short name
  FITC-Conjugated IgG1 Isotype Control
• Technique
  Control, Isotype, FITC, antibody Conjugates, controls, Fluorescein, Isotypes and controls
• Host
  Rat
• Isotype
  IgG1, IgG1
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  fluorecein-coupled Rat IgG1 isoform reference
• Alternative technique
  control, controls, fluorescine
• Tissue
  control

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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