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Estimated Turnaround Time
6-11 Business days
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Target Name
Leukemia Inhibitory Factor
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Target s alterntive name
CDF, D-FACTOR, HILDA, MLPLI, Cholinergic Differentiation Factor, Differentiation-Stimulating Factor, Melanoma-Derived LPL Inhibitor, Emfilermin
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Species Reactivity
Sus scrofa; Porcine (Pig)
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Sample Type
Serum, Plasma, Tissue homogenates and other biological fluids
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Detection Range
31.25-2000 pg/mL
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Sensitivity
11.8 pg/mL
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Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukemia Inhibitory Factor were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukemia Inhibitory Factor were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
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Detection Method
Colorimetric
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Assay Time
3 hours
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Application
For research use only. Not for diagnostic procedures.
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Assay Type
Double-antibody Sandwich
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Shipping Conditions
Ice packs
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Storage Temperature
Short term: 4°C; Long term: see manual.
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Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
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Shelf life
Use Pig Leukemia Inhibitory Factor (LIF) ELISA Kit before 8 months
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Specificity
This assay has high sensitivity and excellent specificity for detection of Leukemia Inhibitory Factor
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Cross Activity
No significant cross-reactivity or interference between Leukemia Inhibitory Factor and analogues was observed.
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Test Principle
No significant cross-reactivity or interference between Leukemia Inhibitory Factor and analogues was observed.
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Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research Area
Cytokine, Tumor immunity, Infection immunity, Neuro science, Bone metabolism
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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About
Pigs and the smaller guinea pigs are frequent used as models for humans.
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Gene target
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Gene symbol
LIF-AS1, LIF-AS2, LIF
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Short name
Leukemia Inhibitory Factor (LIF) ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Pig, Pigs and Guinea pigs
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Alternative name
swine Leukemia Inhibitory Factor (leukemia inhibitory factor) Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
leukemia inhibitory factor, CDF and DIA and HILDA and MLPLI, LIF and IDBG-3968 and ENSG00000128342 and 3976, growth factor activity, Extracellular, Lif and IDBG-142317 and ENSMUSG00000034394 and 16878, LIF and IDBG-636335 and ENSBTAG00000007424 and 280840
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Virus
leukemia
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Name
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Concept
Scope note:
Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.
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Tree numbers
- C04.557.337.372
- C04.619.531
- E05.598.500.496.500
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Qualifiers
diagnosis, parasitology, immunology, microbiology, diagnostic imaging, physiopathology, rehabilitation, enzymology, etiology, mortality, epidemiology, blood, cerebrospinal fluid, chemically induced, congenital, diet therapy, drug therapy, therapy, psychology, urine, virology, history, classification, economics, embryology, genetics, nursing, radiotherapy, ethnology, prevention & control, pathology, metabolism, complications, surgery
