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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser269 of human MAP3K12 (AA266-272)
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Antibody s target
Human MAP3K12 (pSer269)
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Antibody s full description
Rabbit Anti-Human MAP3K12 (pSer269) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
Dlk Antibody, Dual leucine zipper bearing kinase Antibody, Leucine-zipper protein kinase Antibody, M3K12_HUMAN Antibody, MAPK-upstream kinase Antibody, MEKK12 Antibody, Mitogen-activated protein kinase kinase kinase 12 Antibody, MUK Antibody, ZPK Antibody, ZPKP1 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 52.536 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-955 was sufficient for detection of MAP3K12 (pSer269) in 10 µg of human umbilical vein cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Membrane
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Tissue specificity
See included datasheet or contact our support service.
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Scientific context
MAP3K12 is an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_006292
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Gene number
7786
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Protein number
Q12852
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
MAP3K12
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Short name
Rabbit Anti- MAP3K12 (pSer269) Polyclonal: ATTO 680
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
ATTO 680
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman mitogen-activated protein phosphorylation catalyst phosphorylation catalyst phosphorylation catalyst 12 (pSer269) polyclonal: ATTO 680
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
mitogen-activated protein kinase kinase kinase 12, DLK and MEKK12 and MUK and ZPK and ZPKP1, MAP3K12 and IDBG-36895 and ENSG00000139625 and 7786, transferase activity, Plasma membranes, Map3k12 and IDBG-188866 and ENSMUSG00000023050 and 26404, MAP3K12 and IDBG-631263 and ENSBTAG00000020758 and 505427
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