DEFB104A; DEFB4; BD-4; DEFB-4; DEFB104; MGC118942; MGC118944; MGC118945; hBD-4
Beta-defensin 3, Defensin, beta 103, Defensin-like protein, DEFB103A, BD3, DEFB103, DEFB3, DEFB103B
An antimicrobial peptide
Purity by SDS-PAGE
Determined by its ability to chemoattract human monocytes using a concentration range of 0.1-50 ng/ml.
Physical form description
Sterile filtered and lyophilized from 20 mM PBS, pH 7.4 and 130 mM NaCl.
Centrifuge the vial prior to opening. Reconstitute in sterile dd H₂O to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 2-7 days and at -20 °C for future use.
Defensins are cationic peptides with a large spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The Alpha defensins are differentiated from the Beta-defensins by the pairing of their 3 disulfide bonds. Four human Beta-defensins have been identified to date; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence (for BD-1 a propeptide region). Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta Defensin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 50 amino acids. The BD-4 is purified by proprietary chromatographic techniques.
Centrifuge the vial prior to opening.
For Research Use Only! Not to be used in humans
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Recombinants or rec. proteins
BD-4, H. sapiens recombinant