Smad3 (Phospho-Ser425) Antibody

Applications

WB IHC ELISA
Source

Rabbit
Reactivity

H, M, R
MW kDa

48 kDa
Target Modification

Phospho
Modification Sites

H:S425 M:S425 R:S425
Immunogen

The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425.
Immunogen Range

376-425
Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity

Smad3 (Phospho-Ser425) Antibody detects endogenous levels of Smad3 only when phosphorylated at Ser425.
Gene ID

4088
Swiss Prot No

P84022
OMIM No

114500/603109
Unigene No

Hs.618504/Hs.714621
NCBI Gene Symbol

SMAD3
Synonyms

JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3
Storage Stability

Stable at -20°C for at least 1 year.
Physical Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality

Polyclonal
Concentration

1 mg/ml
Properties

If you buy Antibodies supplied by Assay Biotech they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Gene target

Smad3 Phospho-Ser425
Short name

Smad3 (Phospho-Ser425) Antibody
Technique

Antibody, antibodies against human proteins, antibodies for
Alternative name

SMAD family member 3 (phosphorilated-Ser425) (antibody to-)
Alternative technique

antibodies
Alternative to gene target

SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

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