OVA Conjugated Fluorescein Isothiocyanate (FITC)
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Catalog numberCPO843Ge21
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Price:
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Size1mg
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SourceProtein conjugation
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Predicted Molecular Mass KD389.4g/mol
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Accurate Molecular Mass KDNA
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Organism speciesPan-species (General)
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Item NameFluorescein Isothiocyanate
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Alternative NamesFITC-Tag
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Research AreaNA
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Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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TraitsFreeze-dried powder
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Purity> 95%
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Protein lengthNA
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TagN-terminal His Tag
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Expression SystemProtein conjugation
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ApplicationImmunogen; SDS-PAGE; WB.
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PropertiesThis Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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GeneOvalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.
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Gene target
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Short nameOVA Conjugated Fluorescein Isothiocyanate (FITC)
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TechniqueFITC, antibody Conjugates, Fluorescein
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LabelFITC
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SpeciesPan-species (General)
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Alternative nameOVA coupled Fluorescein Isothiocyanate (fluorecein)
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Alternative techniquefluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data