OVA Conjugated Fluorescein Isothiocyanate (FITC)
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Catalog number
CPO843Ge21
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Price
Please ask
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Size
50ug
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Source
Protein conjugation
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Predicted Molecular Mass KD
389.4g/mol
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Accurate Molecular Mass KD
NA
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Organism species
Pan-species (General)
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Item Name
Fluorescein Isothiocyanate
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Alternative Names
FITC-Tag
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Research Area
NA
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Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Traits
Freeze-dried powder
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Purity
> 95%
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Protein length
NA
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Tag
N-terminal His Tag
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Expression System
Protein conjugation
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Application
Immunogen; SDS-PAGE; WB.
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Properties
This Cloud Clone Corp Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cloud Clone Corp FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene
Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein. OVA is also the best characterized and the first antigen proteins used as a transgene to make transgenic mice. Many different transgenic mouse models have systemic OVA expression driven by the ubiquitously expressed b-actin promoter or tissue-specific OVA expression with insulin promoter to drive the transgene expression, for studying type I diabetes, or in different isoforms, secreted or cell-membrane associated, and more recently as inducible transgene models. These C57BL/6 mice, BALB/c mice models are well characterized, and have contributed to our understanding of immunogenicity and tolerance by the OVA model.
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Gene target
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Short name
OVA Conjugated Fluorescein Isothiocyanate (FITC)
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Technique
FITC, antibody Conjugates, Fluorescein
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Label
FITC
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Species
Pan-species (General)
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Alternative name
OVA coupled Fluorescein Isothiocyanate (fluorecein)
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Alternative technique
fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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