HA Affinity Purified Antibody, FITC Conjugated[HA FITC]

Products_type

Antibody
Test

A high affinity purification column was use to purify HA Antibody, FITC Conjugated[HA FITC] by MyBioSource by chromatographic size exclusion.
Description

1 of protein A or G purified by MyBioSource will give more specificity than crude serum.
Properties

If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
French translation

anticorps

Gene target

Conjugated[HA
Gene symbol

MYO1G, KRT31, KRT32, KRT33B, KRT34, KRT35, ARHGAP45, KRT33A, AKAP13, PUM3
Short name

HA Antibody, FITC Conjugated[HA FITC]
Technique

affinity, Antibody, purified, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, purifications
Host

Goat
Label

FITC
Alternative name

HA protein enriched (antibody to-), fluorecein coupled[HA fluorecein]
Alternative technique

pure, antibodies, fluorescine

Identity

HGNC:13880
Gene

MYO1G
Long gene name

myosin IG
Synonyms
- HA-2
Synonyms name
- minor histocompatibility antigen HA-2
GenBank acession
- AF380932
Locus

7p13
Discovery year

2000-11-28
Entrez gene record

64005
Classification
- Myosin heavy chains, class I
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000155821

Identity

HGNC:17102
Gene

ARHGAP45
Long gene name

Rho GTPase activating protein 45
Synonyms gene
- HMHA1
Synonyms gene name
- histocompatibility (minor) HA-1
Synonyms
- KIAA0223
- HA-1
GenBank acession
- D86976
Locus

19p13.3
Discovery year

2005-12-06
Entrez gene record

23526
Pubmed identfication
- 9820596
- 9039502
Classification
- Minor histocompatibility antigens
- AH/BAR family Rho GTPase activating proteins
- F-BAR domain containing

Identity

HGNC:371
Gene

AKAP13
Long gene name

A-kinase anchoring protein 13
Synonyms gene
- LBC
Synonyms gene name
- lymphoid blast crisis oncogene
- A kinase (PRKA) anchor protein 13
Synonyms
- Ht31
- BRX
- AKAP-Lbc
- c-lbc
- PROTO-LB
- HA-3
- ARHGEF13
GenBank acession
- M90360
Locus

15q25.3
Discovery year

1999-09-16
Entrez gene record

11214
Pubmed identfication
- 9627117
- 1860836
RefSeq identity
- NM_007200
Classification
- A-kinase anchoring proteins
- Dbl family Rho GEFs
- Minor histocompatibility antigens
- Pleckstrin homology domain containing
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000172205

Identity

HGNC:29676
Gene

PUM3
Long gene name

pumilio RNA binding family member 3
Synonyms gene
- KIAA0020
Synonyms gene name
- KIAA0020
Synonyms
- XTP5
- PEN
- PUF6
- hPUF-A
- HA-8
- Puf-A
Synonyms name
- penguin homolog (Drosophila)
- minor histocompatibility antigen HA-8
GenBank acession
- AL832239
Locus

9p24.2
Discovery year

2004-01-06
Entrez gene record

9933
Pubmed identfication
- 7584026
- 7584028
- 21266351
RefSeq identity
- NM_014878
Classification
- Pumilio homology domain containing
- Minor histocompatibility antigens
VEGA ID

OTTHUMG00000019450

Name

Fluorescent Antibody Technique
Concept

Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data