Goat anti-Human IgE (epsilon chain) - Affinity Pure, FITC Conjugate

• Catalog number
  MBS687203
• Price
  Please ask
• Size
  1 mg

• Products_type
  Secondary Antibody
• Products_short_name
  [IgE (epsilon chain)]
• Products_name_syn
  [Goat anti-Human IgE (epsilon chain) - Affinity Pure]
• Other_names
  [immunoglobulin E heavy chain, partial]
• Reactivity
  Human. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins light chains on all human immunoglobulins
• Specificity
  Based on IEP, this antibody reacts with: heavy chains on Human IgE
• Purity
  Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Human IgE.
• Form
  Clear, fluorescent yellow liquid
• Concentration
  1.50 mg/ml (E 1% at 280 nm = 13.0)
• Storage_stability
  Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
• Tested_application
  Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
• Test
  A high affinity purification column was use to purify Goat anti- IgE (epsilon chain) - Pure, FITC Conjugate by MyBioSource by chromatographic size exclusion. MyBioSource supplies antibodies that are for research of human proteins.
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti- IgE (epsilon chain) - Pure, FITC Conjugate supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
• Conjugation
  Anti-FITC Antibody
• Latin name
  Capra aegagrus hircus

• Gene target
  epsilon   chain   Conjugate  
• Short name
  Goat anti- IgE (epsilon chain) - Pure, FITC Conjugate
• Technique
  anti-Human, affinity, anti-, anti, FITC, Goat, Pure, IgE, antibody to, antibodies, antibodies against human proteins, Fluorescein, goats, IgEs, pure products
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  IgE, IgE
• Label
  FITC
• Species
  Human, Goats, Humans
• Alternative name
  caprine Antibody toHuman immunoglobuline E (epsilon epitope) - protein Pure, fluorecein Conjugate
• Alternative technique
  antibodies, purified, caprine, fluorescine, immunoglobulin-e

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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