Goat anti Human IgE epsilon chain rhodamine

  • Catalog number
    43R-1138
  • Price
    Please ask
  • Size
    1 mg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Purified Human IgE
  • Raised in
    Goat
  • Specificity
    This antibody reacts with heavy chains on human IgE (epsilon chain).
  • Cross Reactivity
    No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins or serum proteins from bovine, mouse or rabbit IgG1.
  • Conjugate Tag
    Rhodamine
  • Grade Purity
    Grater than 95% pure
  • Method of Purification
    Affinity purified using solid phase Human IgE
  • Source
    Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
  • Concentration
    1.0 mg/ml (lot specific)
  • Form Buffer
    Lyophilized from 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2. Dilute in dH2O to a concentration of 1 mg/ml
  • Storage
    Store at 2-8 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    FC; IF; IHC
  • Usage Recommendations
    The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
  • Latin name
    Capra aegagrus hircus
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
  • Gene target
  • Short name
    Goat anti IgE epsilon chain rhodamine
  • Technique
    anti human, anti, Goat, IgE, antibody to, anti human antibodies, goats, IgEs
  • Host
    Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
  • Isotype
    IgE, IgE
  • Label
    Rhodamine
  • Species
    Human, Goats, Humans
  • Alternative name
    Goat anti Human IgE (epsilon chain) secondary antibody (rhodamine)
  • Alternative technique
    antibodies, caprine, immunoglobulin-e
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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