Rabbit Anti-Mouse IgG (H+L)-FITC
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Catalog numberMBS674900
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PricePlease ask
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Size1 mg
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Products_typeSecondary Antibody
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Products_short_name[Rabbit Anti-Mouse IgG (H+L)]
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Products_name_syn[Rabbit Anti-Mouse IgG(H+L) - FITC; Rabbit Anti-Mouse IgG (H+L)]
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ClonalityPolyclonal
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ReactivityMouse
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SpecificityReacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, and with the light chains of mouse IgM and IgA as demonstrated by ELISA and flow cytometry.
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PurityAffinity chromatography on pooled mouse IgG covalently linked to agarose.
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FormFluorescein (FITC) Conjugate
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Storage_stabilityThe purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
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Tested_applicationELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Oryctolagus cuniculus
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Gene target
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Gene symbolCALD1, FUT1, L3MBTL2
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Short nameRabbit Anti-Mouse IgG (H+L)-FITC
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TechniqueRabbit, anti-, Mouse, anti, FITC, IgG, antibody to, antibodies, Fluorescein, IgGs, mouses
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HostRabbit, Rabbits
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IsotypeIgG, IgG
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LabelFITC
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SpeciesMouse, Mouses
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Alternative nameproduction species: rabbit Antibody toMouse Immunoglobulin G (heavy + light chain)-fluorecein
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Alternative techniquerabbit-anti, murine, antibodies, fluorescine, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namecaldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 1 (H blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
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Synonyms name
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Locus
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Discovery year1989-06-06
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Entrez gene record
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameL3MBTL histone methyl-lysine binding protein 2
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Synonyms gene name
- l(3)mbt-like 2 (Drosophila)
- L3MBTL2, polycomb repressive complex 1 subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-04-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MBT domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
