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Available ordering format
Lyophilized
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Immunogen
to be determined
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Raised in
N/A
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Clonality
N/A
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Clone
N/A
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 225 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS11 1789 | anti-FtsH1-11 | ATP-dependent zinc metalloprotease FtsH1-11AS16 3930 | anti-FtsH1 + FtsH5 | ATP-dependent zinc metalloprotease FtsH1 + FtsH5 (chloroplastic)AS16 3929 | anti-FtsH2 + FtsH8 | ATP-dependent zinc metalloprotease FtsH2 + FtsH8 (chloroplastic)AS07 204 | anti-FtsH3 + FtsH10 | ATP-dependent zinc metalloprotease FtsH3 + FtsH10 (mitochondrial)AS07 205 | anti-FtsH4 | ATP-dependent zinc metalloprotease FtsH4 (mitochondrial)AS05 094A | anti-FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)AS06 130 | anti-FtsH9 | ATP-dependent zinc metalloprotease FtsH9 (chloroplastic)AS07 251 | anti-FtsH10 | ATP-dependent zinc metalloprotease FtsH10 (mitochondrial)Antibodies to other proteins involved in photosynthesisPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a FtsH2 signal in this range.Positive control: load per well: a 2μl load is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
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Molecular weight expected аpparent
75 kDa
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
Concentration: after adding 225 µl of sterile milliQ water final concentration of the standard is 0.1 pmoles/µlThis standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
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References
to be added when available, antibody released in October 2011.
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Scientific context
FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.
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Notes
The FtsH2 protein standard can be used in combination with anti-FtsH2 antibodies to quantitate FtsH2 from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequences in the FtsH protein.Quantitative western blot: detailed method description, video tutorial
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
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Group
positif
