Acid Sphingomyelinase Assay Kit II (Colorimetric)

• Catalog number
  K192-100
• Price
  Please ask
• Size
  100 assays

• Description
  Complete kit that allows the measurement of Acid Sphingomyelinase in biological samples
• Detection Method
  Colorimetric (OD= 570 nm)
• Species Reactivity
  All
• Applications
  Measurement of ASMase activity in various tissues/cell extracts
• Sample Type
  Cell lysate,Serum,Tissue lysate
• Features Benefits
  Complete set of reagents for the detection of Acid SMase in biological samples,Simple, specific (detect acid SMase activity only)
• Storage Conditions
  -80°C
• Shipping Conditions
  Dry Ice
• Shelf life
  12 months
• Background
  Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyelinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Acid   Sphingomyelinase   Kit  
• Short name
  Acid Sphingomyelinase Assay Kit II (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Acid Sphingomyelinase test reagent II (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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