Other name LIPG, Endothelial lipase
Aa sequence Ser 21 – Pro 500
NCBI number Q9Y5X9
Molecular weight This protein is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 55.4 kDa. The predicted N-terminus is Ser 21. DTT-reduced Protein migrates as 55-65 kDa in SDS-PAGE due to glycosylation.
Source HEK 293 cells
NCBI gene LIPG
Target Endothelial Lipase
Storage condition -20°C
Level of endotoxin<1.0 EU per μg of the protein by the LAL method.
Binding ability N/A
Gene source Human
Tested applications SDS-PAGE, N/A
Notes For Research Use Only! Not to be used in humans
Tested activity N/A
Background Lipase endothelial (LIPG) is also known as Endothelial lipase (EL/EDL). This protein has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. LIPG is designated a member of the triglyceride (TG) lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. LIPG mediates both HDL binding and uptake, and the selective uptake of HDL-CE, independently of lipolysis and CLA-1. LIPG also mediates binding and uptake of plasma lipoproteins via a process that is independent of its enzymatic activity, requires cellular heparan sulfate proteoglycans, and is regulated by ligand clustering. LIPG may have unique functional roles in the pathogenesis of coronary artery diseases such as atherosclerosis as well as in lipid metabolism in the vessel wall.
Before use Centrifuge the vial prior to opening.
Shipping under Gel Pack
NCBI gene number 9388
Physical properties Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Biological activity N/A
Physical appearance Lyophilized
Synonym LIPG, Endothelial lipase
How to reconstitute Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins