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Also known as
CD30
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Other names
CD30 antigen; Tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily member 8; CD30 antigen; Ki-1 antigen; CD30L receptor; OTTHUMP00000001783; OTTHUMP00000221481; cytokine receptor CD30; lymphocyte activation antigen CD30; tumor necrosis factor receptor superfamily, member 8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30
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Category
Secondary Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
TNFRSF8; TNFRSF8; CD30; Ki-1; D1S166E; CD30; D1S166E
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Gene name synonims
TNFRSF8; TNFRSF8; CD30; Ki-1; D1S166E; CD30; D1S166E
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Other gene names
TNFRSF8; TNFRSF8; CD30; Ki-1; D1S166E; CD30; D1S166E
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Clonality
Monoclonal
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Immunoglobulin isotype
Armenian Hamster IgG kappa
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Clone
2SH12-5F (also known as mCD30.1)
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Host organism
Armenian Hamster
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Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Murine CD30, Mr 52 kDa (core peptide) CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins.^1,2 Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A.^1 In anti-CD3 epsilon -activated spleen cells, CD30 is expressed primarily on the surface of CD8^+ T cells with peak expression on days 4 and 5. Stimulation of CD30^+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN- gamma production. ^1 While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Fluorescein (FITC) Conjugate
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Concentration
N/A
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Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested applications
Flow Cytometry
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
