Active HIV-2 Protease Recombinant (GST-tagged)

• Catalog number
  7851-20
• Price
  Please ask
• Size
  20 ug

• Synonyms
  HIV-2 retropepsin, HIV-2 Protease (PR2), cd05482
• Alternative_names
  HIV-2 retropepsin, HIV-2 Protease (PR2), cd05482
• Description
  ≥85% Pure Active Recombinant HIV2 Protease (GST tagged) for inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous such applications.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥85%
• Assay
  SDS-PAGE
• Biological activity
  BioVision’s Active HIV-2 Protease has been tested for its activity using HIV-2 Protease Activity Assay Kit (Fluorometric) (K845-100).
• Molecular Weight
  38.3 kDa (1-99 aa + N-terminal GST and C-terminal Poly-his tags). It runs at ~31.5 kDa during SEC and SDS-PAGE analyses.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  6 months
• Concentration
  5 mg/ml
• Appearance
  Liquid
• Physical form description
  5 mg/ml in 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol
• Background Information
  HIV-2 Protease, an aspartyl protease (retropepsin), is essential for the life-cycle of HIV-2 sub-type virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is auto-catalytically released after formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients, the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure. The mutation of HIV protease's active site or inhibition of its activity disrupts HIV’s ability to replicate and infect additional cells. HIV-2 has been found to be less pathogenic than HIV-1. The mechanism of HIV-2 is not clearly defined, nor the difference from HIV-1, however the transmission rate is much lower in HIV-2 than HIV-1.
• Amino acid sequence
  1-99 aa + N-terminal GST and C-terminal Poly-his tags
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Gene
  The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Active   HIV-2   Protease   GST-tagged  
• Gene symbol
  MGST3, HIVEP2, MIR7-2, MIR329-2, MIR512-2, MIR521-2, MIR1289-2, MIR509-2, KRTAP13-2, RNU6-2
• Short name
  Active HIV-2 Protease Recombinant (GST-tagged)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  functionnal human immunodeficiency virus-2 Protease Rec. (GST-tagged)
• Alternative technique
  rec
• Virus
  hiv

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:4921
• Gene
  HIVEP2
• Long gene name
  HIVEP zinc finger 2
• Synonyms gene name
  • human immunodeficiency virus type I enhancer-binding protein 2
  • human immunodeficiency virus type I enhancer binding protein 2
• Synonyms
  • MBP-2
  • HIV-EP2
  • MIBP1
  • ZAS2
  • Schnurri-2
  • ZNF40B
• Synonyms name
  • c-myc intron binding protein 1
• GenBank acession
  • M60119
• Locus
  6q24.2
• Discovery year
  1992-10-21
• Entrez gene record
  3097
• Pubmed identfication
  • 1733857
  • 2022670
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000015713

Gene info

• Identity
  HGNC:31639
• Gene
  MIR7-2
• Long gene name
  microRNA 7-2
• Synonyms gene
  • MIRN7-2
• Synonyms
  • hsa-mir-7-2
• Locus
  15q26.1
• Discovery year
  2004-04-23
• Entrez gene record
  407044
• RefSeq identity
  • NR_029606
• Classification
  • MicroRNA MIR7 family

Gene info

• Identity
  HGNC:32051
• Gene
  MIR329-2
• Long gene name
  microRNA 329-2
• Synonyms gene
  • MIRN329-2
• Synonyms
  • hsa-mir-329-2
• Locus
  14q32.31
• Discovery year
  2005-07-11
• Entrez gene record
  574409
• RefSeq identity
  • NR_029968
• Classification
  • MicroRNA MIR329 family

Gene info

• Identity
  HGNC:32091
• Gene
  MIR512-2
• Long gene name
  microRNA 512-2
• Synonyms gene
  • MIRN512-2
• Synonyms
  • hsa-mir-512-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574459
• RefSeq identity
  • NR_030181
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:32113
• Gene
  MIR521-2
• Long gene name
  microRNA 521-2
• Synonyms gene
  • MIRN521-2
• Synonyms
  • hsa-mir-521-2
• Locus
  19q13.42
• Discovery year
  2005-07-15
• Entrez gene record
  574481
• RefSeq identity
  • NR_030203
• Classification
  • MicroRNA MIR515 family

Gene info

• Identity
  HGNC:35282
• Gene
  MIR1289-2
• Long gene name
  microRNA 1289-2
• Synonyms gene
  • MIRN1289-2
• Synonyms
  • hsa-mir-1289-2
• Locus
  5q31.1
• Discovery year
  2008-11-03
• Entrez gene record
  100302134
• RefSeq identity
  • NR_031621
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:33641
• Gene
  MIR509-2
• Long gene name
  microRNA 509-2
• Synonyms gene
  • MIRN509-2
• Synonyms
  • hsa-mir-509-2
• Locus
  Xq27.3
• Discovery year
  2007-10-24
• Entrez gene record
  100126301
• RefSeq identity
  • NR_030586
• Classification
  • MicroRNA MIR506 family

Gene info

• Identity
  HGNC:18923
• Gene
  KRTAP13-2
• Long gene name
  keratin associated protein 13-2
• Synonyms
  • KAP13-2
• GenBank acession
  • AP001708
• Locus
  21q22.11
• Discovery year
  2003-02-21
• Entrez gene record
  337959
• Pubmed identfication
  • 12359730
• Classification
  • Keratin associated proteins
• VEGA ID
  OTTHUMG00000057793

Gene info

• Identity
  HGNC:34270
• Gene
  RNU6-2
• Long gene name
  RNA, U6 small nuclear 2
• Synonyms
  • U6-2
• Locus
  19p13.3
• Discovery year
  2008-06-10
• Entrez gene record
  103625684
• Pubmed identfication
  • 12711679
• RefSeq identity
  • NR_125730
• Classification
  • Small nuclear RNAs

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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