Description Cell Meter PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* is used as a sensitive probe for flow cytometric analysis of cells that are undergoing apoptosis.
Shipping Blue Ice
Shelflife 6 months
Chemical components Component A: RPE-Annexin V ; Component B: Assay Buffer N/A N/A ; Component C: 100X Nuclear Red™ DCS
Excitation Wavelength 565 nm
Emission Wavelength 575 nm
Personal precautions Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
Uses Laboratory chemicals *For Research Use Only*
Ordering To order Cell Meter PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*, please use the Cat. Nr.22838 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Tips Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*
Additional description This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. aat has more cell death kits and antibodies.This 1 is suited for programmed cell-death studies.For cells, cell lines and tissues in culture till half confluency.FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors.
Test aat supplies other types of Assays as 1.Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
Short name Meter PE-Annexin V Binding Apoptosis Assay Kit *Optimized for *
Technique Flow Cytometry, apoptosis, Assay, Flow
Alternative name cellular Meter peroxidase-Annexin V Binding programmed cellular destruction test reagent *Optimized to measure Flow Cytometry*
Alternative technique celldeath
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832