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Description
Cell Meter Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry* is used to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential(MMP). It is optimized for screening apoptosis activators and inhibitors with a flow cytometer.
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Shipping
Room Temperature
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Storage
Freezer
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Shelflife
6 months
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Chemical components
Component A: 500X MitoTell™ Orange in DMSO ; Component B: Assay Buffer
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Excitation Wavelength
546 nm
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Emission Wavelength
575 nm
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Personal precautions
Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
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Uses
Laboratory chemicals *For Research Use Only*
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Ordering
To order Cell Meter Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*, please use the Cat. Nr.22804 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*
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Test
aat supplies other types of Assays as 1. Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
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Additional description
For cells, cell lines and tissues in culture till half confluency. FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors. Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.