ECHS1, human recombinant

• Catalog number
  P1191-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH, Enoyl-CoA hydratase 1, Short-chain enoyl-CoA hydratase
• Alternative_names
  Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH, Enoyl-CoA hydratase 1, Short-chain enoyl-CoA hydratase
• Description
  Member of the hydratase/isomerase superfamily that funtions in the mitochondrial fatty acid beta-oxidation pathway.
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  > 95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its ability to hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.
• Biological activity
  Specific activity is > 150 units/mg
• Results
  > 150 units/mg
• Unit Definition
  One unit is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C
• Molecular Weight
  30.6 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol, 100 mM NaCl
• Background Information
  Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ECHS1  
• Gene symbol
  ECHS1
• Short name
  ECHS1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  ECHS1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:3151
• Gene
  ECHS1
• Long gene name
  enoyl-CoA hydratase, short chain 1
• Synonyms gene name
  • enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
  • enoyl-CoA hydratase, short chain, 1, mitochondrial
• Synonyms
  • SCEH
• Synonyms name
  • short chain enoyl-CoA hydratase
• Locus
  10q26.3
• Discovery year
  1996-12-17
• Entrez gene record
  1892
• Pubmed identfication
  • 8012501
  • 25393721
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000019320

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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