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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L)]
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Products_name_syn
[Goat anti-Human IgG (H&L) - Affinity Pure]
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Reactivity
Human. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins rat IgG
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on Human IgG, light chains on all Human immunoglobulins
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Human IgG.
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Form
Clear, colorless liquid
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Concentration
1.50 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
ELISA (EIA), Immunohistochemistry (IHC)
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Test
A high affinity purification column was use to purify Goat anti- IgG (H L) - Pure, Conjugate, min x w/ IgG by MyBioSource by chromatographic size exclusion. MyBioSource supplies antibodies that are for research of human proteins.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti- IgG (H L) - Pure, Conjugate, min x w/ IgG supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus, Rattus norvegicus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Short name
Goat anti- IgG (H L) - Pure, Conjugate, min x w/ IgG
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Technique
anti-Human, affinity, anti-, anti, Goat, Pure, IgG, antibody to, antibodies, antibodies against human proteins, goats, IgGs, pure products
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, Heavy & Light chains, IgG
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Label
Biotin
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Species
Rat, Goats, Humans, Rats
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Alternative name
caprine Antibody toHuman Immunoglobulin G (heavy + light chain) - protein Pure, biotinilated Conjugate, min x w/rat Immunoglobulin G
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Alternative technique
antibodies, purified, caprine, immunoglobulins
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