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Available ordering format
Liquid
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Immunogen
Purified Human IgM, µ chain
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other anti-human secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Human IgM (µ chain)
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Goat anti-Human IgM (µ chain), ALP conjugated, is a secondary antibody, conjugated to ALP, which binds to human IgM (µ chain) in immunological assays
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Notes
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Human IgM.Antibody is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on human IgM . Based on IEP, no reactivity is observed to: - non-immunoglobulin human serum proteins - light chains on all human immunoglobulins - human IgG - bovine, goat, mouse, or rabbit serum proteins .
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Protein number
AAC37537.1
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TAIR number
Refer to NCBI
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Latin name
Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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Gene target
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Gene symbol
PGAP1, PGAP3, MPPE1
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Short name
Goat, IgG; , goat, mouse rabbit serum proteins
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Technique
Rabbit, Mouse, serum, Goat, IgG, goats, IgGs, mouses
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgG, IgG
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Species
Mouse, Bos Taurus genes are available form the Bovine Genome database. Goats, Mouses
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Alternative name
caprine, Immunoglobulin G; bovine, caprine, mouse and production species: rabbit blood serum proteins
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Alternative technique
rabbit-anti, sera, murine, caprine, immunoglobulins
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Tissue
serum
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