Goat anti Bovine IgG

• Catalog number
  41C-CB0136
• Price
  Please ask
• Size
  5 mg

• Category
  Secondary Antibody
• Antibody Subtype
  Purified Secondary Antibodies
• Area of research
  Immunology
• Type of Immunogen
  Goat anti-bovine IgG was raised in goat using bovine IgG F(ab')2 fragment ..
• Raised in
  Goat
• Specificity
  Bovine IgG
• Cross Reactivity
  Should be determined by the end user
• Method of Purification
  Goat anti Bovine IgG was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
• Concentration
  5.0 mg/ml
• Form Buffer
  0.02M K3PO4, pH 7.2, 0.15M NaCl, 0.01% NaN3.
• Storage
  Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; IHC; WB
• Usage Recommendations
  ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Latin name
  Capra aegagrus hircus
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Short name
  Goat anti IgG
• Technique
  anti, Goat, IgG, antibody to, goats, IgGs
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Isotype
  IgG, IgG
• Species
  Bovine, Bos Taurus genes are available form the Bovine Genome database. Goats
• Alternative name
  Goat anti Bovine IgG secondary antibody
• Alternative technique
  antibodies, caprine, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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