Myeloperoxidase (MPO) Colorimetric Activity Assay Kit

• Catalog number
  K744-100
• Price
  Please ask
• Size
  100 assays

• Description
  Sensitive Assay, HTS
• Summary
  • Detection method- Absorbance (412 nm) • Species reactivity- Mammalian
• Sample Type
  Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.
• Features Benefits
  • Simple procedure • Fast and convenient • Sensitive assays for measuring MPO as low as 0.05 mU per well
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. MPO contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. MPO catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H₂O₂) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). MPO also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In BioVision’s MPO Assay Kit, the HClO produced from H₂O₂ and Cl- is reacted with taurine to generate the taurine chloramine, which subsequently reacts with the TNB2- probe to eliminate color (λ = 412 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of MPO. This kit can be used to detect MPO as low as 0.05 mU per well.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Myeloperoxidase   MPO   Activity   Kit  
• Gene symbol
  MPO
• Short name
  Myeloperoxidase (MPO) Colorimetric Activity Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Myeloperoxidase (myeloperoxidase) colorimetry Activity test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:7218
• Gene
  MPO
• Long gene name
  myeloperoxidase
• Locus
  17q22
• Discovery year
  2001-06-22
• Entrez gene record
  4353
• VEGA ID
  OTTHUMG00000178922
• Locus Specific Databases
  • MPObase: Mutation registry for Myeloperoxidase deficiency
  • LRG_84

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