Myeloperoxidase (MPO) Peroxidation Activity Assay Kit
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Catalog number
K747-100
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Price
Please ask
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Size
100 assays
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Description
Highly Sensitive Assay, HTS
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Summary
• Detection method- Fluorescence (Ex/Em 535/587 nm) • Applications- • Measurement of MPO activity in various tissues/cells • Screening MPO inhibitors for drug development
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Detection Method
Fluorescence (Ex/Em 535/587 nm)
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Applications
• Measurement of MPO activity in various tissues/cells • Screening MPO inhibitors for drug development
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Sample Type
• Animal tissues: Liver, Heart, Spleen, etc. • Cells: Adherent or suspension
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Features Benefits
• Quick and Easy protocol • Includes specific MPO Inhibitor [4-Aminobenzonic Hydrazide] to delineate MPO activity from total peroxidase activity in the sample. • Highly Sensitive assay for measuring MPO less than 2 μU per well
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Myeloperoxidase (MPO: EC 1.11.2.2) is a peroxidase abundantly expressed in neutrophil granulocytes. It catalyzes the hydrogen peroxide (H2O2) dependent oxidation of chloride anions (Cl-) to generate hypochlorite (HClO). During respiratory burst, neutrophils utilize MPO activity to exhibit cytotoxicity against tumor cells and pathogenic microorganisms. MPO also oxidizes various substances such as phenols and anilines, which can lead to inflammation. Increased MPO levels are associated with an increased risk of cardiovascular disease and myocardial infarction. Thus measurement of MPO peroxidation activity is crucial for disease prediction and therapeutics. BioVision’s Myeloperoxidase (MPO) Peroxidation Activity Assay Kit, oxidizes a substrate to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to total peroxidase activity in the sample. A specific MPO inhibitor is provided, which suppresses peroxidase activity due to MPO. This permits differentiation of MPO-mediated peroxidation from the activity of other peroxidases which may be present in the samples. Our kit provides a sensitive, quick and easy method for detecting MPO activity. The assay is high-throughput adaptable and can detect less than 2 µU of MPO activity.
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Test
Biovision supplies other types of Assays as 1.
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Gene target
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Gene symbol
MPO
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Short name
Myeloperoxidase (MPO) Peroxidation Activity Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
Myeloperoxidase (myeloperoxidase) Peroxidation Activity test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
myeloperoxidase
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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VEGA ID
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Locus Specific Databases
Product images
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