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Also known as
FLAG (DYKDDDDK) Tag
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Other names
N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2b
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Clone
FG4R
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Host organism
Mouse (Mus musculus)
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Species reactivity
FLAG tagged fusion proteins; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Protein A/G Chromatography
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Form Appearance
10mM PBS, pH 7.2, with 0.05% Sodium Azide
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Concentration
N/A
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Storage and shipping
Product should be stored the antibody should be stored at -20 degrees Celsius.. Aliquot to avoid freeze/thaw cycles
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Tested applications
Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS)
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
