Purine Nucleoside Phosphorylase Activity Assay Kit (Colorimetric)

• Catalog number
  K768-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection: Absorbance (OD 293 nm) • Application: Detection of Purine Nucleoside Phosphorylase activity in variety of samples
• Detection Method
  Absorbance (OD 293 nm)
• Applications
  Detection of Purine Nucleoside Phosphorylase activity in variety of samples
• Sample Type
  • Purified recombinant protein • Cell and tissue lysate
• Features Benefits
  • Rapid, simple & convenient • Limit of quantification is 0.1 µU recombinant Purine Nucleoside Phosphorylase.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Purine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allow the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme. PNP deficiency disease leads to toxic buildup of deoxyguanosine in T-cells leading to T-lymphocytopenia with no apparent effects on B-lymphocyte function. Inhibition of PNP is an important target for chemotherapeutic applications and treatment of T-cell mediated autoimmune diseases. PNP deficiency is also associated with neurological problems. In BioVision’s Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed by the breakdown of inosine is further converted to uric acid using a developer. The uric acid is measured at a wavelength of 293 nm. Limit of quantification is 0.1 µU recombinant Purine Nucleoside Phosphorylase.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Purine   Nucleoside   Phosphorylase   Activity   Kit  
• Short name
  Purine Nucleoside Phosphorylase Activity Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Purine Nucleoside Phosphorylase Activity test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Urine Specimen Collection
• Concept
  Scope note: Methods or procedures used to obtain samples of URINE.
• Tree numbers
  • E01.370.225.998.762
  • E05.200.998.762
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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