Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)

• Catalog number
  K767-100
• Price
  Please ask
• Size
  100 assays

• Description
  The most sensitive kit on the market
• Summary
  • Detection: Fluorescence (Ex/Em = 535/587 nm) • Application: Detection of Purine Nucleoside Phosphorylase activity in variety of samples
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm)
• Applications
  Detection of Purine Nucleoside Phosphorylase activity in variety of samples
• Sample Type
  • Purified recombinant protein • Cell and tissue lysate
• Features Benefits
  • Rapid, simple & convenient • Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Purine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme. PNP deficiency disease leads to toxic buildup of deoxyguanosine in T-cells leading to T-lymphocytopenia with no apparent effects on B-lymphocyte function. Inhibition of PNP is an important target for chemotherapeutic applications and treatment of T- cell mediated autoimmune diseases. PNP deficiency is also associated with neurological problems. In BioVision’s Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Purine   Nucleoside   Phosphorylase   Activity   Kit   Fluorometric  
• Short name
  Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Purine Nucleoside Phosphorylase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Urine Specimen Collection
• Concept
  Scope note: Methods or procedures used to obtain samples of URINE.
• Tree numbers
  • E01.370.225.998.762
  • E05.200.998.762
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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